Bactericidal activity of a sanitary and hygienic product based on Bacillus subtilis bacteria strains

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene.

Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder.

Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10⁸ CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 10⁹ CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding.

Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)