EXPERIMENTAL EVALUATION OF CHANGES IN NEUROTROPHIC AND APOPTOTIC FACTORS IN THE IMPLEMENTATION OF LONG-TERM CONSEQUENCES OF ACUTE SEVERE POISONING BY SODIUM THIOPENTAL

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Abstract

The influence of long-term effects of acute poisoning by neurotoxicants on changes in the concentration of neurotrophic factors and factors of apoptosis in laboratory animals has been experimentally investigated. Acute sodium thiopental poisoning was modeled by a single intraperitoneal injection at a dose of 85 mg / kg. In one month after the poisoning the surviving animals were euthanized to select biological material. Changes in concentration of neurotrophic markers: MBP, NSE, BDNF and PEDF in blood serum of laboratory animals and apoptotic factors in brain tissues: caspase 3, caspase 9 and protein p53 have been estimated by ELISA and immunohistochemistry, respectively. The results showed impaired homeostasis of brain neurotrophic factors studied in serum, as well as induction of apoptotic reactions studied in brain tissues of laboratory rats. These changes are one of the pathogenetic links of the mechanism of damage to the nervous system, which can be manifested in violation of higher nervous activity, including changes in behavioral reactions and cognitive functions.

About the authors

T. A. Kostrova

Institute of Toxicology of the Federal Medical-Biological Agency

Author for correspondence.
Email: tasia.pcma.le4@mail.ru

Kostrova Taisiya Aleksandrovna

192019, Saint Petersburg

Russian Federation

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