Evaluation the effectiveness of test-systems for rapid implementation of sanitary-bacteriological analysis of water

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Abstract

There was executed the assessment of the method of the detection and identification of sanitary-indicative microorganisms in water samples with the use of test systems IDEXX manufactured by IDEXX Laboratories, Inc (USA), based on the use of chromogenic culture media and reagents in accordance with the methodology developed by the manufacturer. IDEXX method was found not to be inferior to the reference methods of water analysis applied in Russia and in international practice. Comparative evaluation of crops showed the consistency of results, as in the study of contaminated water with natural microbiocenoses and water modeling of water bodies, contaminated by daily cultures test microganisms. The method allows reduce time of an analysis due to the exclusion of the biochemical reactions (oxidase activity, fermentation of carbohydrates, formation of indole, etc.) needed to determine species of microorganisms, and to obtain the final result of the analysis after 24 hours. On the basis of obtained results test systems IDEXX Laboratories (USA) may be applied on the territory of the Russian Federation for the assessment of water quality according to sanitary-microbiological indices

About the authors

L. V. Ivanova

Center for Strategic Planning and Management of Biomedical Health Risks

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

T. Z. Artemova

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

Angelica V. Zagaynova

Center for Strategic Planning and Management of Biomedical Health Risks

Email: milkbacterialab@list.ru

MD, PhD, Associate Professor, Head of the Laboratory of Sanitary Bacteriology and Parasitology of the Center for Strategic Planning and Management of Biomedical Health Risks , Moscow, 119991, Russian Federation.

e-mail: milkbacterialab@list.ru

Russian Federation

E. K. Gipp

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

T. N. Maksimkina

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

A. V. Krasnyak

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

S. A. Shustova

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

N. A. Yurtseva

Center for Strategic Planning and Management of Biomedical Health Risks

Email: noemail@neicon.ru
Russian Federation

L. M. Raykovich-Bleyzer

IDEXX Laboratories

Email: noemail@neicon.ru
Russian Federation

Zh. Sips

IDEXX Laboratories

Email: noemail@neicon.ru
Russian Federation

S. Kherterikh

IDEXX Laboratories

Email: noemail@neicon.ru
Russian Federation

B. Bruin

IDEXX Laboratories

Email: noemail@neicon.ru
Russian Federation

S. N. Gubin

Ltd «Simedika Ru»

Email: noemail@neicon.ru
Russian Federation

N. A. Bryznyuk

Ltd «Simedika Ru»

Email: noemail@neicon.ru
Russian Federation

References

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  2. GOST R 31955–2012 (ISO 9308-1:2000). «Drinking water. Detection and quantification of Escherichia coli and coliform bacteria. Part 1. Method of membrane filtration». Moscow; 2012. (in Russian)
  3. SanPiN 2.1.4.1074–01. «Drinking water. «Hygienic requirements for water quality of centralized drinking water supply systems. Quality control». Moscow; 2001. (in Russian)
  4. MUK 4.2.1.1018–01. «Sanitary-microbiological analysis of drinking water». Moscow; 2001. (in Russian)
  5. MUK 4.2.1884–04. «Sanitary-microbiological and sanitary-parasitological analysis of surface water bodies water». Moscow; 2004. (in Russian)
  6. MU 2.1.4.1184–03. «Methodical instructions on the introduction and application of Sanitary and Epidemiological Regulations and Norms SanPiN 2.1.4.116–02 «Drinking water. Hygienic requirements for the quality of water packaged in a container. Quality control». Moscow; 2003. (in Russian)
  7. Zavarzin G.A., ed. The Determinant of Berdzhi. Volume 1 [Opredelitel’ Berdzhi. Tom 1]. Moscow; 1997. (in Russian)
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  9. Platonov A.E. Statistical Analysis in Medicine and Biology [Statisticheskiy analiz v meditsine i biologii]. Moscow: RAMS; 2000. (in Russian)
  10. Savilov E.D., Mamontova L.M., Astaf’ev V.A., Zhdanova S.N. The Use of Statistical Methods in Epidemiological Analysis [Primenenie statisticheskikh metodov v epidemiologicheskom analize]. Moscow; 2004. (in Russian)
  11. Khalafyan A.A. Statistical Analysis of Data. Statistics 6 [Statisticheskiy analiz dannykh. Statistika 6]. Moscow: Binom; 2008. (in Russian)

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Copyright (c) 2024 Ivanova L.V., Artemova T.Z., Zagaynova A.V., Gipp E.K., Maksimkina T.N., Krasnyak A.V., Shustova S.A., Yurtseva N.A., Raykovich-Bleyzer L.M., Sips Z., Kherterikh S., Bruin B., Gubin S.N., Bryznyuk N.A.


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