Abstract
There are given results of experimental research on the development of the high-sensitive and selective chromato-mass-spectrometric methodfor the detection of the N-nitrosamines (N-nitrosodimethylamine andN-nitrosodiethylamine) in blood with the use of distillation and automated system of solid-phase extraction Sepaths (Italy) on the cartridges Coconut 6 cm3 at the stage of sample preparation. Chromato-mass-spectrometric analysis of the extract allowed the detection of the completeness of extraction of N-nitrosodimethylamine from blood equal to 98.5% and for N-nitrosodiethylamine - to 100%.The developed chromato-mass-spectrometric method for the detection of N-nitrosamines in biological media (blood) allows the execution of the control of contents of N-nitrosodimethylamine and N-nitrosodiethylamine in the concentration range of 0.002 - 0.1 mg/dm3 at error not more than 27%. The usage of the developed method for the detection of blood N-nitrosamines levels in groups of the observed child population consuming drink water with different content of nitrates allowed to determine a verified difference and excess of N-nitrosamines content by 2.8 times in the group with higher nitrates content in the drinking water as compared to the group of observed cases who drink water with standard content of nitrates. In order to confirm the presence of the defined N-nitrosamines in the blood samples of the people under research there was executed chromato-mass-spectrometric identification in the SCAN mode. Mass spectrums of N-nitrosodimethylamine and N-nitrosodiethylamine discovered in blood samples of observed cases were compared to mass spectrums of NIST 08.L library. The results of identification of N-nitrosodimethylamine and N-nitrosodiethylamine in the blood samples showed that determined compounds have the same structure as the ones given in the library. The method can be used for biomonitoring, sanitary-epidemiological examinations, and researches in evidentiary medicine in order to determine the impact of the unfavourable anthropogenic factors.