Chromatographic Determination of Ibuprofen in Rhodococcus Culture Media and Kinetic Modeling of Its Biodegradation Process

E. V. Vikhareva; Вихарева Е. В.; A. A. Selyaninov; Селянинов А. А.; G. A. Bazhutin; Бажутин Г. А.; E. A. Tyumina; Тюмина Е. А.

doi:10.31857/S0044450223010140

Chromatographic Determination of Ibuprofen in Rhodococcus Culture Media and Kinetic Modeling of Its Biodegradation Process

Authors: Vikhareva E.V.¹^,2, Selyaninov A.A.¹, Bazhutin G.A.³, Tyumina E.A.³
Affiliations:
1. Perm National Research Polytechnic University
2. Perm State Pharmaceutical Academy
3. Perm Federal Research Center, Ural Branch, Russian Academy of Sciences
Issue: Vol 78, No 2 (2023)
Pages: 187-192
Section: ORIGINAL ARTICLES
Submitted: 31.01.2025
URL: https://freezetech.ru/0044-4502/article/view/650178
DOI: https://doi.org/10.31857/S0044450223010140
EDN: https://elibrary.ru/CELDRE
ID: 650178

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Abstract

The conditions for the chromatographic determination of ibuprofen in culture media of Rhodococcus spp. by reversed-phase HPLC are chosen. The specificity, linearity, and accuracy of the developed procedure are confirmed by the validation procedure, which allows its use for a reliable assessment of ibuprofen concentration in the process of bacterial degradation. With the use of kinetic modeling, a forecast is made for changes in the concentration of ibuprofen; the half-life period, the end time of the biodegradation process, and its reproducibility are determined.

Keywords

ibuprofen, HPLC, Rhodococcus, biodegradation, kinetic modeling

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