Karyotype of the black sea scallop (Flexopecten glaber ponticus, Bivalvia, Pectinidae)

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Abstract

Morphometric characteristics of the chromosomes, both meiotic and mitotic, in the eggs and embryos of the Black Sea scallop, Flexopecten glaber ponticus (Bucquoy, Dautzenberg & Dollfus, 1889) were studied using the aceto-orcein staining method. Both metaphase I and metaphase II of the meiosis were established to show 14 bivalents in each. The scallop karyotype consists of 28 chromosomes: two pairs of large subtelocentric (pairs 1–2), three pairs of submetacentric (pairs 3–5) and nine pairs of metacentric chromosomes (pairs 6–14). The average length of homologous chromosomes varied from 1.87 to 5.30 µm. The total length of the haploid set of chromosomes was 44.90 µm. The results obtained allow for chromosome pairs of the Black Sea scallop to be identified, contributing to the construction of an idiogram suitable for a comparison with the karyotype of the Mediterranean scallop, F. glaber.

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About the authors

А. V. Pirkova

A. O. Kovalevsky Institute of the Biology of the Southern Seas, Russian Academy of Sciences

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Email: avpirkova@mail.ru
Russian Federation, Sevastopol

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2. Fig. 1. Meiosis of Flexopecten glaber ponticus: A – metaphase I in unfertilized and B – metaphase II in fertilized eggs (14 bivalents, the arrow indicates the 1st direction body). Scale bar is 2 µm.

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3. Fig. 2. Flexopecten glaber ponticus: A – metaphase of mitosis in an embryonic cell, B – homologous chromosomes of the karyotype. Scale bar is 2 µm.

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4. Fig. 3. Flexopecten glaber ponticus: idiogram constructed according to the data in Table 1.

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5. Fig. 4. Correlation between the relative length of chromosomes (Lr,%) and the centromeric index (Ic,%) of the karyotype of Flexopecten glaber ponticus.

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6. Fig. 5. Functional hermaphrodite Flexopecten glaber ponticus. A–C – gonads of scallops with different volumes of the ovary located in the distal part; D – gonad of the scallop with two lobes of the ovary located in the proximal and distal parts; 1 – lobe of the ovary; 2 – lobe of the testis.

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