Biochemical and molecular-genetic growth indices of rainbow trout (Oncorhynchus mykiss Walb.) in the conditions of the southern region of the Russian Federation in a long-term study

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Abstract

The expression of genes encoding transcriptional myogenic regulatory factors (MRF) in muscles, and the activity of enzymes involved in energy and carbohydrate metabolism in the liver and muscles of rainbow trout Oncorhynchus mykiss Walb., grown under 24-hour lighting and fed at night in aquaculture conditions in one of the southern regions of the Russian Federation for seven months, were studied. The results of the study showed that this regime, introduced into the standard technology of trout growing, had a beneficial effect on its condition and growth. By the end of the study, trout individuals grown under the experimental regime differed from the control variants in having higher expression levels of the MRF genes (myod1, myf5, mlc2, mstn1a) and increased activity of pyruvate kinase and cytochrome c oxidase in the liver. It can be assumed that the introduction of additional lighting and feeding at night into the technology of trout cultivation stimulates aerobic metabolism, promotes the intensification of muscle growth processes in trout individuals and, in general, has a positive effect on the fish organism condition.

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About the authors

M. A. Rodin

Federal Research Center “ Karelian Research Center of the Russian Academy of Sciences”

Author for correspondence.
Email: mikhail.rodin.mr@yandex.ru

Institute of Biology

Russian Federation, st. Pushkinskaya, 11, Petrozavodsk, 185910

M. V. Kuznetsova

Federal Research Center “ Karelian Research Center of the Russian Academy of Sciences”

Email: mikhail.rodin.mr@yandex.ru

Institute of Biology

Russian Federation, st. Pushkinskaya, 11, Petrozavodsk, 185910

M. Yu. Krupnova

Federal Research Center “ Karelian Research Center of the Russian Academy of Sciences”

Email: mikhail.rodin.mr@yandex.ru

Institute of Biology

Russian Federation, st. Pushkinskaya, 11, Petrozavodsk, 185910

A. E. Kuritsyn

Federal Research Center “ Karelian Research Center of the Russian Academy of Sciences”

Email: mikhail.rodin.mr@yandex.ru

Institute of Biology

Russian Federation, st. Pushkinskaya, 11, Petrozavodsk, 185910

N. N. Nemova

Federal Research Center “ Karelian Research Center of the Russian Academy of Sciences”

Email: mikhail.rodin.mr@yandex.ru

Institute of Biology

Russian Federation, st. Pushkinskaya, 11, Petrozavodsk, 185910

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2. Fig. 1. Average monthly temperature.

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3. Fig. 2. Average weight and specific growth rate (numbers on graphs) of trout individuals grown under different lighting and feeding regimes.

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4. Fig. 3. Enzyme activity in the liver of trout individuals grown under different lighting and feeding regimes. LDH activity is presented as a value/20. * – differences between experimental groups are significant, p < 0.05.

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5. Fig. 4. Enzyme activity in the muscles of trout individuals grown under different lighting and feeding conditions. CO activity is presented as a value*200. * – differences between experimental groups are significant, p < 0.05.

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